Pin1 activity assay

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August undefined, 2024

WebDec 20, 2024 · Pin1 is a peptidyl-prolyl isomerase responsible for isomerizing phosphorylated S/T-P motifs. Pin1 has two domains that each have a distinct ligand binding site, but only its PPIase domain has catalytic activity. WebJul 2, 2001 · Pin1 levels were determined by immunoblotting analysis, as in (A), and semi-quantified using Imagequant. Actin was used as an internal control, and the Pin1 level in each sample was expressed as the Pin1:actin ratio. ( D) Comparison of Pin1 levels in mammary epithelial cell lines.

SensoLyte Green Pin1 Activity Assay Kit Fluorimetric

WebJan 30, 2024 · The general understanding of Pin1 in human cancer is that it promotes cancer occurrence and development by activating numerous oncogenic genes and … WebApr 1, 2024 · Isomerase assays confirmed this and revealed complete and very rapid suppression of Pin1 activity due to Ser16 phosphorylation by PKCζ or PKMζ. 32 We also showed that under basal conditions, Pin1 interacted with hyperphosphorylated eIF4E, 4E-BP1, and 4E-BP-2, providing a molecular link to translation initiation and regulation. While … myfox weather live

Tumor suppressive activity of prolyl isomerase Pin1 in renal cell ...

WebIn the present disclosure, elevated active monomeric Pin1 levels may be detected by Pin1 biomarkers, which may include Pin1 Q33K or E100D … WebAug 29, 2024 · Pin1 is the only known peptidyl-prolyl cis–trans isomerase (PPIase) that specifically recognizes and isomerizes the phosphorylated Serine/Threonine-Proline … WebFeb 1, 2024 · Pin1 (protein interacting with never in mitosis A-1) is a member of the peptidyl prolyl isomerase (PPIase) family, and catalyzes cis-trans isomerization of pThr/Ser-Pro … myfox weather.com

Recombinant Human Cyclophilin A Assay: R&D Systems

Structural insights into auxin recognition and efflux by Arabidopsis PIN1

WebDec 12, 2024 · Using co-immunoprecipitation and CDK kinase activity assays, we found that PIN1 binds the phosphorylated Thr 187 –Pro motif in p27 and reduces p27's interaction with cyclin A– or cyclin E–CDK2, leading to increased CDK2 kinase activity. ... For CDK2 kinase activity assay, co-immunoprecipitation of cyclins A/E–CDK2 and p27 was performed ... WebTumor suppressive activity of prolyl isomerase Pin1 in renal cell carcinoma. Mol Oncol.. 2011-10; 5 (5):465-74. Teng BL, Hacker KE, Chen S, Means AR, Rathmell WK. a Department of Pharmacology and Cancer Biology, Duke University, 308 Research Drive, Durham, NC 27710, USAb Lineberger Comprehensive Cancer Center, University of North Carolina at ... ofsted nominee trainingWebDec 12, 2024 · PIN1 is a peptidyl-prolyl isomerase that catalyzes the cis / trans isomerization of peptide bonds between proline and phosphorylated serine/threonine residues. By … ofsted northampton international academy

"WebAug 10, 2024 · (E) The PPIase activity assay of Pin1 incubated with the Pin1 inhibitor API-1, with an IC50 of 72.3 nM (red), and juglone, with an IC50 of 7.68 lM (blue). Graphic data were run in triplicate and ... " - Pin1 activity assay

Pin1 activity assay

WebTwo hit compounds are identified, which after further structure optimization have the potential to be developed as antitumor drugs and show the anticancer activity of HWH8-33 and H WH8-36 against Pin1 for the first time. Background: Pin1 is a member of the evolutionarily conserved peptidyl-prolyl isomerase (PPIase) family of proteins. Following … WebChIP assay and dual luciferase were used for assessment of transcriptional activity. Results: Both Pin1 and IL-18 levels are increased in primary PDAC tissues and that their levels are positively correlated. High expression of IL-18 is a predictor of poor prognoses.

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WebSep 22, 2024 · The inhibitory activities of Pin1 inhibitors, developed by the team, were confirmed through in vitro PPIase assay. However, their potential non-specific effects on other PPIase enzymes or kinases ... WebPin1 is a PPIase specific for pSer/pThr-Pro motifs; Uses recombinant human Pin 1 and substrate Succ-AEPF-pNA; Measures cis/trans isomerisation kinetics of substrate using …

WebMounting evidence has demonstrated that Pin1 is widely overexpressed and/or overactivated in cancer, exerting a critical influence on tumor initiation and progression … WebMar 27, 2024 · Multifaceted approaches were taken to determine the inhibition profiles of these compounds against Pin1 activity in vitro and in vivo, including an isomerization assay, surface plasmon resonance (SPR) technology, virtual docking, MTT proliferation assay, western blotting, cell cycle analysis, apoptosis analysis, immunofluorescence analysis ...

WebApr 27, 2015 · Pin1 (Peptidyl-prolyl cis-trans isomerase NIMA-interacting 1; Peptidyl-prolyl isomerase, PPIase) is an enzyme that isomerizes phospho/Threonine-Proline motifs. … WebPin1 activity can be measured in SNs or cell lysates by means of a protease-coupled, cis-to-trans isomerization assay ( 24, 32, 33 ). Conversion of the peptide substrate (Suc-Ala-Glu-Pro-Phe-pNA) from cis to trans permits cleavage of the C-terminal nitroaniline by chymotrypsin and detection at 390 nm.

WebMar 27, 2024 · The activity of Pin1 was determined via a protease-coupled isomer-specific assay using suc-AEPF-pNA peptide (Bachem) as the substrate (Fischer et al., 1989). …

WebJun 25, 2024 · The optical density of each well in the assay, which in proportional to both the LDH activity and percentage of necrotic cells, was measured at a wavelength of 490 or 450 nm with a microplate reader. The percentage of necrotic cell death was calculated from four independent experiments by measuring the optical density of the treated group minus ... myfoxylearningWebEGCG inhibited Pin1 activity in a protease-coupled assay (IC 50 = 20 μM) and suppressed JNK signaling, cyclin D1 and Bcl-xL expression in ErbB2-transformed MEFs (Urusova et al. 2011 ). Tolerable doses of EGCG reduced the growth of ErbB2-transformed MEF xenografts in immune-deficient mice (Urusova et al. 2011 ). ofsted no place for bullyingWebFeb 13, 2024 · Pin1 consists of short N-terminal protein-protein interaction domain that allows enzyme to bind phosphoproteins and longer C … myfpb ccWebPin1 consists of short N-terminal protein-protein interaction domain that allows enzyme to bind phosphoproteins and longer C-terminal izomerase domain. Pin1 has many biological … myfoxtwincities channel 9WebJun 2, 2014 · Specific activity is > 330 nmoles/min/mg, and is defined as the amount of enzyme that cleaves 1 µmole of suc-AAFP-pNA per minute at 25°C in Tris-Hcl pH8.0 using chymotrypsin. Activity Assay. 1. Prepare 180ul assay buffer into a suitable container: 133mM Tris-HCl, pH8.0, 5.5nM chymotrypsin, and 0.5ug of Pin1 recombinant protein. myfoxyjewels.comWebFeb 1, 2024 · The trypsin activity assay kit can detect 10-100 mU (p-NA unit) trypsin. Trypsin activity assay protocol summary: - add samples, positive control and standards to wells. - … ofsted notifiable eventsWebJun 10, 2013 · Pin1 activity assay. g for 10 min (4°C). Pin1 activity was measured using equal amounts of artery cytoplasmic lysates and α-chymotrypsin using a synthetic tetrapeptide substrate Suc-Ala-Glu-Pro-Phe-pNa (Peptides International, Louisville, KY, USA). Absorption at 390 nM was measured using an Ultrospec 2000 spectrophotometer. ofsted notice of inspection