WebImaging of species in living organisms with high spatiotemporal resolution is essential for understanding biological processes. While functional nucleic acids (FNAs), such as catalytic nucleic acids and aptamers, have emerged as effective sensors for a wide range of molecules, photocaged control of these FNAs has played a key role in translating them … WebBoth probes can be efficiently uncaged via irradiation to produce the active C-nucleophile probe DYn-2. Labeling assay in living cells demonstrated DYn-2-ONB exhibited better …

Dr. Eike F. Jöst – Principal Scientist – Merck Life Science LinkedIn

Webvalidate the photorelease of photocaged TAM,22 it required near toxic concentrations (Z8 mM). Consequently, our initial test of ONB–4OHT compounds used similarly high concentrations (5 mM), and we often observed reduced cell viability after treatment (Fig. 1d). Therefore, we sought to re-optimize condi-tions for use with photocaged 4OHT. WebJan 4, 2024 · Recent advances in fluorescence imaging techniques and super-resolution microscopy have extended the applications of fluorescent probes in studying various cellular processes at the molecular level. Specifically, organelle-targeted probes have been commonly used to detect cellular metabolites and transient chemical messengers with … flo gummy vitamin side effects

Photocaged dicarbonyl probe provides spatiotemporal …

WebJun 16, 2024 · Remote activation of biomarker sensing holds a great promise of shifting the success of in vitro diagnostics to spatiotemporally controlled in vivo visualization of tumor, and in turn, imaging guided therapy. Herein, a “dual-key-one-lock” nanodevice was designed and built by assembling thermo-activatable probe of trimeric DNA hybrids into a … WebJan 10, 2011 · The rational design, synthesis, photocontrollable sensing in solution and in living cells, and mechanistic studies of a molecular prototype of PCAFP for copper are reported, which apparently have the intrinsic advantage of spatiotemporal control when compared to traditional fluorescent probes. Analyte-responsive fluorescent probes are … WebEarly cancer diagnosis is of great significance to relative cancer prevention and clinical therapy, and it is crucial to efficiently recognize cancerous tumor sites at the molecular … flo gummy vitamin reviews