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Melatonin mediated Foxp3-downregulation decreases cytokines production ...
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WebNov 1, 2024 · The paraffin blocks were cut into 4-μm sections that were dehydrated with an alcohol gradient, cleared with xylene, and stained with either hematoxylin and eosin, or by the IHC protocol with primary antibodies against TLR2 (ab2068, Abcam), TLR4 (sc-293072, SANTA CRUZ) and Foxp3 (#12653, CST). Webproteins work well with the Foxp3/Transcription Factor Staining Buffer Set (cat. no. 00-5523), while secreted proteins such as cytokines and chemokines work well with the Intracellular Fixation & Permeabilization Buffer Set (cat. no. 88-8824). Lastly, there are several phosphorylated signaling proteins WebFOXP3 is a 47 kD transcription factor, also known as Forkhead box protein P3, Scurfin, JM2, or IPEX. It is proposed to be a master regulatory gene and more specific marker of T regulatory cells than most cell surface markers (such as CD4 and CD25). Transduced expression of FOXP3 in CD4 + /CD25 - cells has been shown to induce GITR, CD103, … chilton shaker furniture